Reemergence of TSWV in a large scale in recent years: What's going on?


University of Georgia

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Southeast (GA, FL, AL)

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ur recent research has shown that partial 
sequences from virus isolates collected in 2010 were different than those collected in the 90s. Despite such differences there was no evidence of the presence of resistance-breaking strains of the virus then. The recent reports of increased incidences of spotted wilt in reliably resistant 
cultivars convinced us to reassess the situation. This project is a multi-year undertaking. My 
PhD student, Ms. Pin-Chu Lai is leading this effort. Ms. Lai completed her MS work at UGA doing TSWV-peanut research. We believe that sequencing the genome completely would be 
ideal to addressing this issue. Though the genome of TSWV is relatively small ( ~ 17KB), there have been some troubleshooting regarding isolating the viral RNA from total plant RNA. Next generation sequencing did not provide the complete coverage of at least one of the RNAs. We have been attempting to address the issue, and are taking numerous other approaches. In the 
meantime, samples from multiple plant cultivars are being collected and stored. We intend to 
accomplish complete sequencing this fall and in the spring of 2018. 

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