Gene-based Markers for Breeding of Nematode and Leaf Spot Resistance

Institution:

Board of Regents of the University System of Georgia on behalf of the University of Georgia Cooperative Extension Service and University of Georgia Research Foundation, Inc.

Budget ID:

1420

Project ID:

407

Report BID:

State:

Georgia

Region:

Southeast (GA, FL, AL)

State Group:

GA-159

Project Fiscal Year:

2016

Category:

Breeding/Genetics/Biotech

Report Type:

Report Received Date:

Investigator:

Ozias-Akins

Project NPB Budget:

$20,000

The research supported by this funding addressed several goals of the Peanut Genomic Initiative Strategic Plan including: Genetic Tools and Breeding Methods, Genomic Sequencing & Gene Discovery, and Functional Genomics & Proteomics. Sequencing of expressed genes is essential to extract the portion of the genome that is largely responsible for the phenotype of the plant. Quantitative analysis of gene expression also allows evaluation of differential gene expression across tissues and between duplicated genes, particularly with respect to whole genome duplications, which is important for understanding the genetic contributions of the subgenomes of tetraploid peanut. We accomplished the objectives to 1) complete gene expression profiling using RNA-Seq for resistant and susceptible genotypes challenged with late leaf spot and previously nematode; and 2) identify additional single nucleotide polymorphisms (SNPs) in differentially expressed genes for genetic mapping in populations segregating for these disease resistance traits. 

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